Tender For Fluorescence Microscope With Live-Cell Imaging, Chennai-Tamil Nadu

Tender For Fluorescence Microscope With Live-Cell Imaging; 1 Inverted Microscope  Bright field, Fluorescence and DIC illumination with accessories  Motorized beam path selection for visual and camera imaging  Motorized Z focus drive with minimum stable resolution of 10nm or better  The microscope should have at least 22 mm FOV both for eyepiece and camera port  6 position or higher motorized FL filter wheel, 6 positions motorized DIC nosepiece  1-1.5X magnification changer  Motorized XY stage for the movement of specimen using ergo joy-stick as well as total control by microscopy software  LED illumination for transmitted light .  IR LED based Z drift compensation system for long term live cell imaging 2 High Resolution Objectives  Plan Fluorite grade objectives N.A 0.13 or better  Plan Fluorite grade objective long working distance 20X N.A 0.45 or better  Plan Fluorite grade objective long working distance 40X N.A 0.6 or better  Plan Apochromat 60x 1.42 N.A 1.42 Oil or better 13 | P a g e 3 LED Illumination : LED Fluorescence Illumination 20,000 hours of life for fluorescence observation ,filter for DAPI/FITC/Texas Red must be offered. 4 Condenser & DIC Attachments: DIC attachment for 20X,40X,60X Objective with analyzer andpolarizer attachment. 5 Software: The system should be quoted with software to control motorized microscope withfollowing features  Time Lapse imaging  Zstack imaging  Mutipoint, Imaging  Multichannel Acquisition  Experimental Preview  Annotations  Image Filters  Morphology  Image Arithmetic  Multi dimensional Image Viewer  Tiling  Max/Min Projection  Ratio and Graphing  Live compare  Binary layers  Snapshot  Moovie  Segmentation  Region of Interest  Time measurement  Colocalization  Interactive movie  Volume measurement  Montage 6 Onstage Incubation System: Onstage Incubation system with Temperature and humidity control for long term live cell imaging 7 Camera Attachment: 20 Megapixel or better Dual mode CMOS camera capable of imaging bothFluorescence and chromogenic dyes, With Speed up to 60 fps or better should be supplied with microscope to capture live samples, fluorescence slides as well brightfield slides stained with chromogenic dyes 8 Vibration Isolator for Microscope: Imported Anti Vibration Platform with High-Precision Air Spring, r Air Compressor / Air Supply, Air Valves for Adjustment, Auto-Leveling With a metal top table must be provided 14 | P a g e 9 Computer Workstation: Intel Xeon Processor (6C 3.8GHz 4.6GHz Turbo HT 8.25MB 130W DDR4- 2933)64GB (4x16GB), DDR4 2933 ECC 512GB NVMe + 2TB SATA 7200rpm NVIDIA RTX A4000 16GB 4DP DVDRW Windows 11 Pro for Workstations (6 cores) or better configuration with 32 Inch HDmonitor, Keyboard and Mouse should be supplied with the system 10 Confocal Scan Head and Detection System The detectors must be separated from the scan head and connected through high transmission optical fiber in order to avoid noise during confocal imaging, Vendors who provides direct mountdetectors must offer chiller or colling options in order to avoid noise during confocal imaging  High transmission efficiency optics for confocal .  At least 2 GaAsP/Hyd having 45% Q.E or better for two color simultaneous and 4 color sequential imaging  Computer controlled continuously variable single pinhole system, which should cover wider area of one Airy unit for higher brightness and without affecting sectioning performance  Scanning Speed imaging capability of 8-10 fps@ 512x 512 and maximum 220 fps @ 512x 16 or better.  Confocal System should have 22 mm FOV imaging capability for capturing large samples and perform multiwell plates .  System should possess efficient dichroic mirror with low angle incidence for better transmission efficiency.  Maximum scan resolution up to 8K x 8K or better.  Scan zoom of 1 – 40x or more continuous variable. Multi step scanning zoom preferred  Transmitted light detector to be provided for capturing bright field and DIC images. 11 Lasers: Pre-aligned Solid state laser launcher with atleast 15-20mw or better laser power:  Solid state 405nm  Solid state 488nm  Solid state 561nm  Solid state 633nm/638nm/640nm 15 | P a g e Other Terms and Conditions 12 TIRF attachment with TIRF laser 488,561 and 640 should be quoted with High NA 100 X TIRFObjective with N.A 1.48 or better 13 Warranty : 24 months 14 Delivery: 4 months from the date of PO 15 Optional Accessories (For Confocal & TIRF which will be placed on the same Microscope)

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INR 130000.0 /-

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