Tender For Nextgen O2k Fluo, hyderabad-Telangana

Tender For Nextgen O2k Fluo; 1. NextGen-O2K Fluo, Series XB: The complete experimental system for high-resolution fluorespirometer enabling simultaneous monitoring of oxygen consumption together with coenzyme Qredox state, NAD(P)H redox state, H2O2production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+uptake capacity (CaG) and ATP production(MgG) 2. NextGen-O2k: Hardware and DatLab Software: Stainless steel housing ; Peltier thermostat 4 to 47 °C; ±0.002 °C; - Barometric pressure transducer; -2 Amperometric oxygen channels ; - 2 ISE sockets for ion sensitive electrodes; - 2 Sockets for optical signals or NO; - 2 Sockets for Q-and NAD(P)HModules; - 2 Sockets for PB-Module; - 2 Duran® glass chambers; - 2 SmartPOS with connector; - 2 PVDF stirrers;, electromagnetic control; - 2 PEEKstoppers with O-ring; - 2 Blue POM chamber holders with O-ring and V-ring; - 2 Blue POMOroboPOS-holders 3. NextGen-O2k: Accessories and Consumables: 1ISS-Integrated Suction System, to remove medium from the O2k; -Chamber 1 Oxia; – the O2 regimecontroller for HyperOxia and HypOxia 1 O2kTitration Set: Hamilton microsyringes; fixedneedles with rounded tips (8 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 100 mm3,1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks 1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL1 Pkg. OroboPOS-Seal Tip 2 Pkg. OroboPOSMembranes 1 OroboPOS-Electrolyte Powder 1OroboPOS-Mounting Tool 1 Polishing Cloth 1 Polishing Powder 0.3 μm 1 O2-Zero Powder 1 Forceps for membrane application 1 Pipette Plastic2 ungraded 1 Pkg. Conditioning cloths for stainless-steel housing4. O2k-Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, membrane mt-potential, Ca2+ - 2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity - 2 Smart FluoSensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity - 3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters 5. TIP2k-Module: add-on for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control - 4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip - 1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool - 1 TIP2k-Needle Safety Support with cable guide - 1 Pkg. TIP2k-Filter Papers (10/Pkg.) 6. O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function - 2 Duran® glass chambers, with smaller operation volume (V) of 0.5 mL - 2 PVDF stirrers for small volume chambers, electromagnetic control - 2 PEEK stoppers for small volume chambers with O-ring - 2 Black POM chamber holders with O-ring and V-ring - 2 Black POM OroboPOS-holders 7. Integrated computer with touch screen – Display (15,6″; FHD 1920×1080) - Pre-installed DatLab connects to NextGen-O2k - Keyboard, webcam and accessories 8. Q-Module: for measuring the Q-redox state and cyclic voltammetry: 4 Q-Stoppers beige PEEK conical shaft side+2.6 mm +1.3 mm central port: with glassy carbon and platinum electrode embedded ; 2 Reference electrodes; 2.4mm Ø glass shaft; 10 Replacement barrels for reference electrodes; 2.4 mm glass ; 1 Electrolyte Reference-Electrode; 1 ISE-Filling Syringe with needle; 1 Stopper-Needle; 2 Polishing Powder 0.3 μm ; 2Polishing Powder 0.05 µm; 4 Polishing Cloth; 3 Pkgs Q-Filter Papers (10/Pkg) ; 2 Boxes O-ring3 Viton® 12.5×1 mm, 8/box. 9. NADH-Module: for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence: 2 NADH-Sensors ; 2 Black PVDF stirrers; 3 Pkg of Filter sets R370 for NADH(12/Pkg) 10. PB-Module: for conducting measurements of PhotoBiology, including photosynthesis: 6 PhotoBiology light sources – PB-Light Sources (2 blue, 2 red and 2 white)

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