Rate schedule of Chemicals & KITsunder WBUAFS-Collagenase Type I 3 CryoXL Stem Cell Freezing Medium 4 HiAdipoXL Adipocyte Differentiation medium 5 HiOsteoXL Osteocyte Differentiation medium 6 HiOsteoXL Chondrocyte Differentiation medium 7 Human Recombinant Interferon- γ 8 Lipopolysaccharides from Escherichia coli O127:B8 9 Luria Bertani Broth, Miller pH 7.0,Granulated 10 Luria Bertani Agar, Miller pH 7.0 ,Granulated 11 Mouse IL-10(Interleukin 10) ELISA Kit 12 Mouse IL-1β(Interleukin 1 Beta) ELISA Kit 13 Mouse IL-6(Interleukin 6) ELISA Kit 14 Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit 15 KnockOut Serum Replacement,100 ml 16 STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit 17 CTS KnockOut DMEM/F-12 18 Geltrex hESC-Qualified,Reduced Growth Factor Basement Membrane Matrix 19 StemFlex Medium 20 Gentamicin (10 mg/mL) 21 Antibiotic-Antimycotic (100X) 22 Lipofectamine 3000 Transfection Reagent 23 Neon™ Transfection System 10 μL Kit 24 dNTP set,100 mM,4X0.25 ml 25 DreamTaq DNA Polymerase,500 unit 26 Revert Aid First cDNA synthesis kit,100 rxn 27 PowerUp SYBR Green Master Mix,5 ml 28 Spectra Multicolor Broad Range Protein Ladder 29 Dnase I, Rnase free- 1000unit 30 100 bp DNA ladder,50 micro gm 31 DEPC,25ml 32 Hydrogen Peroxide; 500 ml 33 pGEM-T Easy Vector Systems 34 CloneJET PCR Cloning Kit, 40 reaction 35 GeneJET Plasmid Miniprep Kit, 50 reaction 36 RNaseZap RNase Decontamination Wipes 37 Glycogen, molecular biology grade 38 Phusion Hign FidelityDNA Polymerase,100 unit 39 Sodium Acetate 40 Millon’s reagent (Mercury) 41 Sodium Nitrite 42 Casein 43 Gelatine 44 Ammonium sulphate 45 Sulphosalicilic acid 46 Benzidine power 47 Ethanol 48 Rectified Spirit 49 Hydrochloric Acid 50 Sulfuric Acid 51 Nitric Acid 52 Heparin 53 Sodium Phosphate Dibase (Na2HPO4) 54 Potassium Phosphate Monobase (KHPO4) 55 Chloroform 56 Isopropanol 57 Agarose 58 Carbinol 59 Formaldehyde LR 60 Methylene blue 61 Lactophenol solution 62 Glycerol 85% AR 63 Giemsa’sstain solution 64 Leishman’sstain 65 Wright stain 66 A-B stain 67 Acid fast stain (Z-N stain) 68 Haematoxylin 69 Eosin 2% 70 Clove oil 71 Acid carmine 72 Borax carmine 73 Acid fast stain/ Z-N stain 74 Buffer Capsules pH 7.2 75 Phosphate Buffer Saline (PBS) pH 7.2 76 Phosphate Buffer Saline (PBS) pH 7.4 77 Chloroform, for molecular work 78 Sodium chloride Molecular grade 79 Sodium hydroxide (NaOH) pallet 80 Potassium hydroxide (KOH) pallet 81 Potassium dichromate 82 DiEthyle Ether/ Ether 83 Immersion oil 84 Xylene AR 85 Acetic acid glacial AR 86 Acetone AR 87 Charcoal powder 88 Clearing solution/ Extran 89 Ethidiun bromide 90 TAE/TBL Buffer 91 DreamTaq DNA Polymerase,500 unit 92 dNTP set,100 mM,4X0.25 ml 93 PowerUp SYBR Green Master Mix,5 ml 94 Spectra Multicolor Broad Range Protein Ladder 95 Dnase I, Rnase free- 1000unit 96 100 bp DNA ladder,50 micro gm 97 DEPC,25ml 98 Hydrogen Peroxide; 500 ml 99 pGEM-T Easy Vector Systems 100 Glycogen, molecular biology grade 101 PhusionHignFidelityDNA Polymerase,100 unit 102 Glycerol 103 TRIZOLE/PureZole 104 Acetone 105 Eosin red 106 Sodium Acetate 107 Millon’s reagent (Mercury) 108 Sodium Nitrite 109 Casein 110 Gelatine 111 Ammonium sulphate (NH4)2SO4 112 Sulphosalicilic acid 113 Benzidine power 114 Ethanol 115 Rectified Spirit 116 Hydrochloric Acid (HCl) 117 Sulfuric Acid(H2SO4) 118 Nitric Acid (HNO3) 119 Heparin 120 Sodium Phosphate Dibase (Na2HPO4) 121 Potassium Phosphate Monobase (KHPO4) 122 Chloroform 123 Isopropanol 124 Agarose Powder Molecular grade 125 1% DMSO (DimethyleSulfoxide) 126 Lymphocyte seperation medium Specific for Lymphocytes and monocytes separation from whole blood 127 Nitrobluetetrazolium chloride (NBT) Cell Culture Tested, 128 Thiazolyl blue tetrazolium bromide (MTT) Cell Culture Tested, 129 Dimethyl sulphoxide (DMSO) Molecular Biology grade, 130 Dulbeccos Phosphate Buffered Saline 1X, Tisue culture grade, without Calcium and Magnesium, 131 RPMI 1640 media, Supplemented withGlutamine,HEPES buffer, Glucose, Sodium bicarbonate without Sodium pyruvate, 132 N,N-Dimethylformamide, Molecular Biology grade, 133 Concanavalin A, Cell culture tested, 134 Cyber green master mixBased on 2-step SYBR green chemistry suitable for Quantification for qPCR, Run time 60 mins, 135 Trizol reagent for RNA extraction 136 Rnase free water (Nuclease free) molecular grade 137 EthidiumBromamide reagent 138 Drabkins reagent(with standard) 139 Leishmans Stain 140 RBC diluting Fluid 141 Sodium citrate 142 WBC diluting Fluid 143 Rectifed sprit 144 Nigrosine 145 Methyl alchol 146 EDTA disodium Salt,Dihydrate extra pure AR, 99.5% 147 Epinephrine extra pure ,98% 148 NaOH,Sodium Hydroxide Pellets Purified,97% 149 H2O2,Hydrogen Peroxide,TMB Solution B (30% H2O2) 150 HCl,Hydrochloric acid abt. 35% pure, Hi-AR™ 151 ECBU4BTC, pH 4.01 Buffer Solution (Red) 152 ECBU7BTC, pH 7.00 Buffer Solution (Yellow) 153 ECBU10BTC, pH 10.01 Buffer Solution (Blue) 154 Sucrose, Hi-ARTM/ACS 155 p-Amino-N,N-dimethylaniline oxalate 156 Andrade Peptone Water 157 0.1% Peptone 158 Phenol Red Indicator 159 Bromothymol Blue Indicator 160 Kovacs’ Indole Reagent 161 Triple Sugar Iron Agar 162 MR-VP Medium 163 Methyl Red Indicator 164 Potassium hydroxide pellets 165 Simmons Citrate Agar 166 1-Naphthol 167 Gelatin Agar 168 Urea Agar Base 169 Ornithine Decarboxylase Broth 170 L-Lysine, Hi-LR™ 171 Blood Agar Base 172 Moeller Decarboxylase Broth Base 173 Mueller Hinton Agar 174 BHI Agar (Brain Heart Infusion Agar) 175 BHI Broth (Brain Heart Infusion Broth) 176 RS Medium Base 177 Agar powder, Bacteriological grade 178 Giemsa’s Stain 179 NO 5 Selective Supplement 180 TCBS Agar 181 Glutamate Starch Phenol Red Agar Base 182 Potassium permanganate 183 EDTA, 1M Solution 184 Propanol, Hi-AR™ 185 Egg Yolk Emulsion 186 Muller Hinton Broth 187 Sodium thiosulphate anhydrous 188 Potassium hydroxide pellets 189 Phenol: chloroform: isoamyl alcohol mixt 190 Chloroform isoamyl alcohol (24:1) 191 Molecular Biology Grade Water 192 Manganese sulphate, monohydrate 193 Starch soluble, Hi-AR 194 Mercuric chloride, Hi-LR 195 Sodium Hydroxide pellets 196 STPP 197 Sucrose 198 D-sorbitol 199 NaHCO3 (99.9%) 200 Sodium hexametaphosphate 201 Bile extract (Bovine) 202 Pancreatin from Porcine 203 Pepsin (1:10000) 204 Deionized water 205 Acetone 206 Egg Yolk Tel Emulsion 207 HCl 208 Bacillus Cereus Agar Base 209 Baired Parker Agar Base 210 Leishman’s solution 211 NaHCO3 Sodium Bicarbonate ACS, 99.7% 212 Na2Co3 Sodium Carbonate anhydrous ACS , 99% 213 EDTA disodium Salt 214 Dihydrate extra pure AR, 99.5% 215 Epinephrine extra pure ,98% 216 Na2HPO4 Disodium Hydrogen Phosphate Diabasic Anhydrous for molecular biology,99.5% 217 NaH2PO4 Sodium dihydrogen Phosphate extra pure AR,99% 218 C6H3N2O4Cl (1-Chloro -2,4 Di-Nitro Benzene (CDNB) extra pure 99% 219 C10H17N3O6S Reduced glutathione (GSH) 99.5% 220 KH2PO4(Potassium Dihydrogen Orthophosphate extra pure AR, 99.5 % 221 NaOH Sodium Hydroxide Pellets Purified,97% 222 H2O2Hydrogen Peroxide 223 TMB Solution B (30% H2O2) 224 C7H16ClNO2Acetylcholine Chloride extrapure,99% 225 NH2OH.HCl Hydroxylamine Hydrochloride pure,98% 226 Trichloroacetic acid (Hi-AR) 227 Thiobarbituric acid (Barbituric acid, Hi-AR) 228 Ferric Chloride Hexahyrate, ACS, 97% FeCl3 229 HCl Hydrochloric acid abt. 35% pure 230 pH 4.01 Buffer Solution (Red) 231 pH 7.00 Buffer Solution (Yellow) 232 pH 10.01 Buffer Solution (Blue) 233 Ferrous ammonium sulphate hexahydrate 234 Sucrose 235 Revert Aid First cDNA synthesis kit,100 rxn 236 CloneJET PCR Cloning Kit, 40 reaction 237 GeneJET Plasmid Miniprep Kit, 50 reaction 238 RNaseZapRNase Decontamination Wipes 239 Promega DNA extraction kit 240 RNA extraction kit 241 Blood and tissues DNA extraction kit 242 cDNA extraction kit 243 RNase inhibitor 244 DNase kit 245 Anti Sheep-IgG Horse raddish Peroxidase conjugate,EIA-grade, 246 Anti Goat-IgG Horse Reddish Peroxidase 247 Anti Rabbit-IgG Horse Reddish Peroxidase 248 ELISA plate (NUNC), 96 well multiwall plate, 249 Syringe filter, 0.2um 250 Cell Culture Insert 6 well plate, 0.4 micron, Semi-transparent 251 Cell Strainer, 70µm 252 Tissue Culture Plates 96 well
