Tender For Annual Rate Contract For Supply Of ‘Media For Ivf Required For Art Centre’ For Department Of Obstetrics & Gynaecology,Aiims Nagpur; 1 Item Name with Specification 2 80% Gradient Density gradient Media used to separate cells, organelles, membrane vesicles, viruses, proteins, nucleic acids, and lipoproteins. Density gradient centrifugation (DGC) is a popular procedure used in Assisted Reproductive Technology (ART) to select sperm based on their density.(Capacity 12 ml Vials) 3 40% Gradient used to separate cells, organelles, membrane vesicles, viruses, proteins, nucleic acids, and lipoproteins. Density gradient centrifugation (DGC) is a popular procedure used in Assisted Reproductive Technology (ART) to select sperm based on their density. (Capacity 12 ml Vials) 4 Sperm wash used to wash sperm and isolate motile sperm using the swim-up method. The ideal sperm preparation medium helps maintain a stable pH and osmolality while providing the proper physiological environment for sperm survival.(50 ml capacity vials) 5 Sperm Freezing Media HEPES-buffered media which also contains physiologic salts, glycine, glucose, lactate, and the cryoprotectants glycerol (15% and 27%). sucrose and human serum albumin (4.0g/l, medicinal substance derived from human blood plasma) to protect the sperm from damage during the freezing procedure.(10 ml vials) 6 Sperm Mobil Spermmobil is a HEPES-buffered reagent containing low bicarbonate. It is used for in-vitro examination of sperms of necrozoospermic ejaculates as well as of immotile sperms isolated from testicular tissue(0.05 ml ) 7 Sperm Morphology Kit Pre stained Slides for Sperm Morphology assessment Spermatozoon is an intricate motile cell with a well-defined head, neck and tail. The shape of the normal sperm is a reflection of proper spermatogenesis in the testicles 8 DNA Fragmentation Kit (Sperm Chroma Kit) Halosperm: An in vitro diagnostic kit that can measure DNA fragmentation without complex laboratory equipment 9 Sperm Vitality Kit The FluoVit kit enables the evaluation of sperm vitality in fresh or thawed semen samples. 10 Semen Fructose Kit A sperm fructose kit, also known as a semen fructose test, measures the amount of fructose in human semen or seminal plasma. Fructose is an energy source for sperm motility and metabolism, and its absence can indicate issues with the seminal vesicles or ejaculatory duct. 11 Sageone step One-step media is a type of culture media used for human embryo culture. The composition of one-step media includes:Magnesium sulfate, Potassium chloride, Potassium phosphate, Sodium chloride, Amino acids, L-alanyl-L-glutamine, Calcium-L-lactate, Glucose, Sodium pyruvate, and Sodium bicarbonate(20 ml vials) 12 Embryo Thawing Kit For thawing of human zygotes and cleavage stage embryos 1 M Sucrose Thawing Medium with 12 mg/ml Human Serum Albumin 0.5 M Sucrose Thawing Medium with 12 mg/ml Human Serum Albumin MOPS 13 Embryo Freezing kit Vitrification Media Kit includes:Equilibration SolutionVitrification Solution 14 HYLASE1ML Vial HYALSE contains 80 IU/mL of Hyaluronidase in HEPES buffered HTF with HSA.For the removal of cumulus and corona cells fromm the oocyte prior to ICSI procedure. 15 PVP 7% 0.5ML Vial -PVP reduces the sperm motility simplifying the immobilization and easing the handling during intracytoplasmic sperm injection (ICSI). It also prevents sperm from sticking to the injection pipette (V-ICSIPET) and gives better control of media and sperms. 16 OIL100ML PET OIL is a ready to use light paraffin oil for ART-procedures and applicable for short and long term cultures up to 6 days. 17 Oocyte freezingkit x 1.5 ml Basic Solution vial (BS) 1 x 1.5 ml Equilibration Solution vial (ES)2 x 1.5 ml Vitrification Solution vial (VS)(10 ml Vials) 18 Oocyte thawing kit Media for oocytes, free from protein, compose of synthetic and plant derivatives, hydroxy propyl cellulose and to be used as a non-protein supplement. Sterility tested. Mouse Embryo Assay tested. Contains Sucrose, Sodium lactate, Sodium Bicarbonate. Antibiotic. Medium is ready to use. Storage at 2-8°C.(10 ml Vials) 19 Oocytes Activation Media. Media use for activation of oocytes. Maintain a stable environment during. Washing oocytes. Antibiotics. Medium is ready to use. Store at 2-8 ْC. Protect from light(10 ml Vials) 20 In Vitro Maturation Media. In Vitro Maturation Media is for the in-vitro maturation of immature oocytes, subsequent fertilization, and embryo development(10ml vials) 21 Laboratory equipment disinfectant disinfectant for medical equipment in in-vitro fertilization (IVF) laboratories, including CO2 incubators and laminar flow hoods. Its a transparent, odorless, ready-to-use, alcohol-free, non-VOC releasing, and non-fragranced mixture.Its effective against bacteria, yeasts, viruses, and mycobactericidal. It doesnt corrode metals or affect organic soil. (2 Litre) 22 Laboratory surface disinfectant Non toxic, non odour, non alcohol, no acetone, no aldehyde. Compatible with metal rubber and plastic. Effective against hepatitis B, HIV, rotavirus with in 1-2 minutes, microbectrium within 4-6 minutes bacteria fungi between 12-17 minutes, Ready to use or dilution. (2 litre) 23 Laboratory Hand disinfectant. Non toxic,safe disinfection for hands. Non alcohol , no odour. Effective against hepatitis B, HIV, rotavirus with in 1-2 minutes, microbectrium within 4-6 minutes bacteria, fungi. Neutralizes bacteria which causes bad smell does not dry the skin. Ready to use. Storage : Room temperature.(2 litre) 24 Embryo glue EmbryoGlue has the basc composition of a rich blastocyst culture medium and contains recombinant albumin and a high concentration of hyaluronan It can be used for transfer of all embryo developmental stages, including cleavage embryos, blastocysts after assisted hatching, biopsied embryos and cryopreservation.(10ml Vials) 25 Double Distilled Sterile water Purified by steam distillation for use as a research laboratory reagent. (5 litre Can) 26 Gamete Buffer retrieval, holding and washing of oocytes is ready-to-use and especially designed for in vivo use during oocyte retrieval. Flush is fully synthetic and does not contain human serum albumin (HSA) or antibiotics. When supplemented with Heparin, the medium is used to prevent clotting if blood is present after flushing the follicles.(100 ML) 27 Fertilization supports both gametes during fertilization and promotes sperm function(20ml) 28 Cleavage formulated to support early embryo development(20ml) 29 Blastocyst designed to meet metabolic needs after embryo genome activation(20ml) 30 Deionised Water Water is passed through a reverse osmosis membrane and a deionizing membrane. Does not conduct electricity. Ultrapurified. 31 70%Iso Propyl Alcohol Isopropyl alcohol (IPA) is effective as a disinfectant issolve more slowly, penetrate cells, and kill bacteria
