Specifications For High Perfomance Liquid Chromatography (Hplc) For The Department Of Clinical Pharmacology, Byl Nair Hospital, Mumbai.-, Mumbai-Maharashtra

Specifications For High Perfomance Liquid Chromatography (Hplc) For The Department Of Clinical Pharmacology, Byl Nair Hospital, Mumbai.-1. Pump a Operating Principle Parallel- type/ or series type double plunger b Gradient formation Quaternary Low-pressure gradient c Flow rate range 0.001-10 ml/min d Flow rate accuracy ±0.1 - ±1.0% e Flow rate precision ≤0.05 - 0.075%RSD f Pressure range 5000 –10,000psi g No. of eluent lines 4 solutions h Solvent degassing Built – in, minimum 4 channels i Safety Measures Liquid leakage sensor j Concentration accuracy 0.50-0.80%RSD k Composition precision ≤0.25% 2. Autosampler injector with cooler a Sample capacity Minimum 100 × 1.5 ml b Sample injection system Loop injection or total volume sample injection/ direct injection c Sample injection volume 0.1 – 100 μl d Injection volume accuracy ±1.0 % e Injection volume precision ≤0.5 % RSD (when 5 is injected) f Carry over of sample ≤ 0.005 – 0.05% with Caffeine/ Chlorhexidine g Sample thermostatting 4 to 400C h Safety Measures Liquid leakage sensor i Injection cycle time ≤20 seconds j No. of samples for processing 100 or more 3. Column Oven a Type of column oven Forced-air-circulation type / Peltier type b Temperature range 10–85℃ or more c Temperature Accuracy ± 0.1℃ d Temperature precision ≤ 0.2℃ e Column capacity Minimum 2 columns f Safety Measures Liquid leakage sensor 4. Fluorescence detector a Data acquisition ≥70 HZ b Light Source Xenon lamp, minimum life of 2000 working hours c Wavelength Range Excitation -200 -650nm Emission -250-700 nm d Spectral Bandwidth 20nm e Wavelength Accuracy ±3nm f Safety Measures Liquid leakage sensor 5. Photo Diode Array (PDA) detector a Provision of Photo diode array Yes b PDA detector Wavelength range in nm 190 – 800 or better c No. of diodes 1024 d PDA detector light source Deuterium and Tungsten lamps e PDA detector wavelength accuracy ±1nm f Data rate in Hz 100 g Noise in AU Less than 0.8 x 10-5 h PDA detector Drift in AU/hour Less than 0.5 x 10-3 i Temperature controlled flow cell in 0C 50C above room temp or 500C 6. Column a Type of column C-18 b No. of columns 4 c Dimensions of column 250mm x 4.6mm x 5μ

Refer document

Refer document

Refer document