Tender For “Lab Equipment” And “Chemicals, Glassware, Plastic Ware And Miscellaneous Lab. Items”. 1. Technical Specifications of Microprocessor pH Meter Sl. No. Feature Specifications 1 pH Range 0 to 14.00 pH 2 pH Resolution 0.1 pH / 0.01 pH 3 pH Accuracy ± 0.01 pH 4 Calibration upto 3 points 5 pH buffer recognition USA (pH 4.01, 7.00, 10.01) 6 Temperature compensation 0 to 100 °C Auto/Manual 7 mV Range +1999.9 mV 8 mV Resolution 1 mV 9 mV Accuracy ± 1 % FS + 1 digit 10 Temperature Range 0 to 100°C 11 Auto-Off Manual or Automatic (10, 20, 30 minutes after last key 6 pressed(Selectable) 12 Display LCD 13 Power supply DC9V, using AC adapters, AC 220V / 50Hz 14 Dimensions LxWxH (mm) 160 × 190 × 170 mm 15 Weight 750g 16 Replacement warranty 3Yr 17 Pre Installation requirements (if any) 18 AMC/extended warranty after warranty period (if any) 2. Technical Specifications of Submarine Electrophoresis Sl. No. Feature Specifications 1 Principal Material Polycarbonate 2 Size 310 x 150 x 90 (mm) 3 Comb thickness 1.0, 1.5 and 2.0 (mm) 4 Buffer volume About 650 mL 5 Gel dimension(L x W) 120 x 120 (mm) 120 x 60 (mm) 60 x I20 (mm) 60 x 60 (mm) 6 No. of Platinum electrodes red and black (1 each) 7 No. of samples Runs up to 13, 18, 25 Samples 8 Gel scoop 1 No. 9 Weight 1 KG 10 Replacement 3Yr warranty 11 Pre Installation requirements (if any) 12 AMC/extended warranty after warranty period (if any) 3. Technical Specifications of Table Top Cooling Centrifuge Sl. No. Feature Specifications 1 Motor Type DC brushless motor 2 Safety Performance Dual door lock, over-speed detection, over-temperature detection with Internal diagnosis system. 3 Fast Spin Yes, Calibration window on lid for speed 4 Display LED 5 Acceleration and deceleration time 25s↑25s↓ 6 Rotor imbalance detection alarm Yes 7 Temperature display °C 8 Run Time 30s-99 min; HOLD (continuous running) 9 Nett Weight 30kg 10 Max. Speed 15,000rpm (200-15,000rpm) in 100rpm increments 11 Max. RCF 21,380×g, step: 10×g 12 Program storage capacity 9 13 Rotor head Rotors with 0.2mL, 0.5mL, 1.5mL, 2mL and 5mL centrifuge tubes/ PCR tubes capacity 14 Max. Capacity 1.5/2mL×24, 0.5mL×36, PCR8×4, 8 5mL×12, 5mL×18 15 Temperature setting -20℃- 40℃ 16 Noise _56dB 17 Power Requirements Single-phase, 200V-240V, 50Hz/60Hz, 500W 100V-120V, 50Hz/60Hz, 500W 18 Dimensions(mm) 332(Width) × 553(depth) × 283(height) 19 Other functions Rotation speed/RCF switchover, inching operation, running process indicator, sound alert. Acceleration/ deceleration in 9 steps, program storage capacity. 20 Replacement warranty 3Yr 21 Pre Installation requirements (if any) 22 AMC/extended warranty after warranty period (if any) 4. Technical Specifications of Orbital shaking Incubator Sl. No. Feature Specifications 1 Temperature Range & Accuracy R.T.+5 ~60°C , ± 0.1°C 2 Environment Cooling and heating both 3 Flask Capacity 100 ml x 4 / 250 ml x 2/ 500 ml x 1 4 Temperature Control Accuracy@37°C +0.3°C 5 Clamps Lotus clamp Plastic (Adjustable for different flask capacity)/ Lotus clamps for 250/500 ml capacity. (Lotus Clamp 1000ml Optional) 6 Shaking Platform Size 18 x 18 7 Orbital Motion Diameter 12 mm 8 Shaking Speed range (RPM) 50~200rpm 9 Capacity 100 Ltr 10 RPM Display LED Display 11 RPM Controller Speed Regulator 12 Exterior Powdered coated steel sheet 13 Insulation PUF 14 Air circulation Motor driven blower 15 Heating Tubular air heater 16 Cooling CFC free refrigeration system 17 Temperature controller Microprocessor PID controller Display of SV & PV 18 Temperature sensor PT100 19 Door Solid door with magnetic rubber gasket (Observation window) 20 Illumination LED light 21 Power Supply 220 Volt /50 Hz 22 Tray 1 Removable tray to keep petri dishes 23 Replacement warranty 3Yr 24 Pre Installation requirements (if any) 25 AMC/extended warranty after warranty period (if any) 10 5. Technical Specifications of PCR thermal cycler Sl. No. Feature Specifications 1 Capacity 96x0.2ml(PCR plate without skirt or with semi skirt) 12x8x0.2ml strips,8x12x0.2ml strips, 0.2ml tubes(height 20~23mm) 2 Automatic Heating Lid Heating lid turns off automatically, once the temperature of the samples is lower than30ºC or the program ends 3 Max. Heating Rate 4.5ºC/S 4 Max. Cooling Rate 4.0ºC/S 5 Block Temp. range 4~99.9ºC 6 Timer 1s~99min59sec/Infinite 7 Block Temp. accuracy ±0.2ºC 8 Block Temp. Uniformity ±0.25ºC 9 Avg. Heating Rate 3ºC/s 10 Avg. Cooling Rate 2.5ºC/s 11 Temperature control via Block or Tube 12 Gradient Max30ºC, Accuracy±0.3ºC 13 Gradient Temp. Accuracy ±0.3ºC 14 Block Temp. Accuracy 0.1ºC 15 Temp. Range of Heating Lid RT+30ºC~105ºC 16 Temp. Holding Temp:16ºC Duration: infinite 17 Timer In/decreasing -599~599S for long PCR 18 Temp. In/decreasing -9.9~9.9ºC for Touchdown PCR 19 Programs >100 20 Max.Cycles 99 21 Max.Steps 30 22 Program Pause Yes 23 Real-time display Text and image 24 USB Port USB 2.0 25 Dimension and weight 200x300x170(mm) 4.5kg 26 Replacement warranty 3Yr 27 Pre Installation requirements (if any) 28 AMC/extended warranty after warranty period (if any) 6. Technical Specifications of UV-Transilluminator Sl. No. Feature Specifications 1 Flexibility Adjustable intensity 2 View size (mm) 210D x 260W 3 Wavelength (nm) 302 and 365 4 Light source UV tubes:11x 8W 5 Blocking cover Aluminum alloy frame 6 Power 100~240V, 50/60Hz 7 Weight (Kg) 4.3kg 8 Outer dimensions (mm) 280Dx 340Wx 80H 12 9 Replacement warranty 3Yr 10 Pre Installation requirements (if any) 11 AMC/extended warranty after warranty period (if any) 7. Technical Specifications of Electric power backup Sl. No. Feature Specifications 1 Online UPS 1kVa 2 Backup 30mins 3 Replacement warranty 3Yr 4 Pre Installation requirements (if any) 5 AMC/extended warranty after warranty period (if any) iv. Additional features not specified by the University 2. List of Chemicals Sl. No. Chemicals Make Cat. No Pack Size Quantity Required 1. Potato dextrose Agar SRL 71788 500g 1no. 2. Formaldehyde Himedia AS127 500ml 1no. 3. Potato dextrose broth SRL 35208 500g 1no. 4. CTAB Himedia MB101- 500G 500g 1no 5. Tris-HCL Himedia GRM1218 500g 1no 6. β-mercaptoethanol Himedia MB041 250ml 1no 7. RNase A Solution Himedia DS0003 20 mg/ml 1no 8. Proteinase K Himedia DS0013 20 mg/ml 1no 9. Protein Ladder(10-245kDa) Himedia MBT092 10ln 1no. 10. Glycine Himedia PCT0310 500g 1no. 11. EDTA Himedia GRM1195 500g 1no 12. Agarose Himedia RM273 500g 2no 13. Luria broth SRL 14593 500g 1no 14. Nutrient agar SRL 68650 500g 1no 15. Nutrient broth SRL 62811 500g 1no 16. HiPurA® Quick Gel Purification Kit Himedia MB539 1 Kit 2no. 17. Taq DNA pol Sigma- Aldrich D4545- 250UN 250U 2no. 18. DNase free water Sigma- Aldrich W4502-1l 1l 1no. 19. dNTPs Himedia MBT079 0.25ml 1no. 20. Magnesium chloride hexahydrate Himedia GRM728 500 g 1no. 21. 1kb DNA Ladder Himedia MBT051 250μl 1no. 22. Hi-Chrom PCR Master Mix Himedia MBT089 20reac 5no. 23. Ethidium bromide Himedia MB071 5g 1no. 24. Xylene cyanol Himedia RM859 10ml 1no. 25. Glycerol SRL 77453 2500ml 1no. 26. Bromophenol blue Himedia GRM914 5g 1no. 27. Tris base Himedia TC072 500 1no. 28. MS medium Himedia PT045 1l 4no. 29. 6-Benzylaminopurine (BAP) Sigma- Aldrich 200241 1g 1no. 30. 1-Naphthaleneacetic acid (NAA) Sigma- Aldrich N0640 25g 1no. 31. Boric acid Himedia TC580M 500g 1no. 32. Ethanol Himedia MB106 500ml 10no. 33. Methanol SRL AS059 2500ml 1no. 34. TEMED Himedia GRM1572 100ml 1no. 35. Plasmid isolation kit Sigma- Aldrich 1175477700 1 50 rec. 1no. 36. DNA Ligase enzyme Sigma- Aldrich 1163537900 1 1kit 1no. 37. pGEM™-T Easy Vector Systems Fischer scientific PR-A1360 20 reactions 1no. 38. pBI121 plant vector NovoProBios cience V010909 5μg 1no. 39. Acetosyringone Sigma- Aldrich D134406 1g 1no. 40. Primer synthesis Eurofins EGI-DS-023 7no. 41. DNA sequencing Eurofins EGI-DS-007 2no. 42. Calcium chloride Sigma- Aldrich C7902-500g 500g 1no. 43. X-gal Sigma- Aldrich 3117073001 100mg 1no. 44. IPTG, Isopropyl β-Dthiogalactoside Sigma- Aldrich I5502-1g 1g 1no. 14 45. YEB broth Himedia M1263 500g 1no. 46. Guaicol Sigma- Aldrich G5502-100 100g 1no 47. Pholoroglucinol Sigma- Aldrich 79330 25g 1no 48. Ampicillin Himedia SD002 10mg 1no 49. Sodium acetate Himedia GRM1012 500g 1no. 50. Kanamycin Sigma- Aldrich K4000-1 1g 1no 51. DH5α E. coli Thermofisher 1 kit 1no. 52. Rifampicin Sigma- Aldrich 557303 1g 1no. 53. Triton-X 100 Himedia TC286 500 1no. 54. Sucrose Sigma- Aldrich G7021-1 1kg 1no. 55. Cefotaxime sodium salt Sigma- Aldrich C7039 100mg 1no. 56. Agar SRL 85473 500g 1no. 57. Coomassie Brilliant Blue Himedia MB092 250g 1no. 58. Glacial acetic acid Himedia AS119 500ml 1no. 3. List of Glassware 4. List of Plastic Ware Sl. No. Glassware items Make Cat. No Capacity/Size Quantity Required 1. Jam bottle RJ Industry G-12 450 450ml 20no 2. Durran bottle Merk Z305162-10EA 500ml 5 no. 3. Test tubes Borosil 9800U08 25 x 150 25 no. 4. Beakers Borosil 9900018 50ml, 100ml, 500ml 5 each 5. Erlenmeyer, Conical flask Borosil 4980016/49800 21/4980024/498 0029 100 ml, 250ml, 500ml, 1l 5 each Sl. No. Plastic ware item Make Cat No. Capacity/Size Quantity Required 1. Cryo box Tarson 524000 1 & 1.8ml 3no. 2. Micro Centrifuge Tubes Himedia PW146- 1X500NO 1.5ml and 2ml 1pk 45. YEB broth Himedia M1263 500g 1no. 46. Guaicol Sigma- Aldrich G5502-100 100g 1no 47. Pholoroglucinol Sigma- Aldrich 79330 25g 1no 48. Ampicillin Himedia SD002 10mg 1no 49. Sodium acetate Himedia GRM1012 500g 1no. 50. Kanamycin Sigma- Aldrich K4000-1 1g 1no 51. DH5α E. coli Thermofisher 1 kit 1no. 52. Rifampicin Sigma- Aldrich 557303 1g 1no. 53. Triton-X 100 Himedia TC286 500 1no. 54. Sucrose Sigma- Aldrich G7021-1 1kg 1no. 55. Cefotaxime sodium salt Sigma- Aldrich C7039 100mg 1no. 56. Agar SRL 85473 500g 1no. 57. Coomassie Brilliant Blue Himedia MB092 250g 1no. 58. Glacial acetic acid Himedia AS119 500ml 1no. 3. List of Glassware 4. List of Plastic Ware Sl. No. Glassware items Make Cat. No Capacity/Size Quantity Required 1. Jam bottle RJ Industry G-12 450 450ml 20no 2. Durran bottle Merk Z305162-10EA 500ml 5 no. 3. Test tubes Borosil 9800U08 25 x 150 25 no. 4. Beakers Borosil 9900018 50ml, 100ml, 500ml 5 each 5. Erlenmeyer, Conical flask Borosil 4980016/49800 21/4980024/498 0029 100 ml, 250ml, 500ml, 1l 5 each Sl. No. Plastic ware item Make Cat No. Capacity/Size Quantity Required 1. Cryo box Tarson 524000 1 & 1.8ml 3no. 2. Micro Centrifuge Tubes Himedia PW146- 1X500NO 1.5ml and 2ml 1pk 15 5. List of Miscellaneous Lab Consumables Sl. No. Item Make/Cat No. (if any) Capacity/Size Quantity Required 1. Whatman Filter paper 1001-917 460x570 Sheets 2 pk 2. Micropipette Tarson 0.5-2μl, 1-10μl, 2- 20μl, 20-200μl, 200-1000μl 4no. 3. Pipettor Stand Tarson 5 place 1no. 4. Hi Indicator pH Paper LA335-1PK/Himedia Range : pH 1 to 14 2 pk 5. Metaloop -SS-2* LA650-1X8NO 2.2 mm dia. 8 pcs 6. Thermometer 1C/Labtex -20 to +150⁰C 1no. 7. Sterile Forceps PW1337- 1X10NO/Himedia 12.5 cm, 6 pcs 8. Gloves LA910- 5X20NO/Himedia Medium 3 boxes 9. Tissue paper ICA stella/ 1012 900 of 23 cm 20 pcs 10. Cotton (Absorbant) 4681029 500g 10 pcs 11. Cotton (Nonabsorbant) 9413156 500g 10 pcs 12. Mortar pestle CH11607/2 170mm 4 pairs 13. Parafilm LA017-5NO/Himedia 2” x 250’ on 1” dia. core. 5pk 14. Autoclavable Bags PW057-250NO/Himedia 11”(h) x 7”(b), 1.0 kg 20no.
