Tender For Supply, Installation & Commissioning Of Multimodal Plate Reader With Imaging No. Name & Specification Qty Rate inclusive all taxes, GST etc Multimodal plate reader with imaging 1 The multimode microplate reader and imaging systems can be integrated/single or separate systems also Multimode Microplate Reader: The instrument should be a spectral scanning multimode microplate reader capable of doing photometry, Fluorometric Intensity, Luminescence, Alpha Screen and AlphaLISA and upgradable to hTRF. Auto Gain facility should be available, not the default settings in software. Instrument should automatically calibrate results with different gain settings to obtain single consistent measurement range. Self diagnostic option and auto-calibration during the starting of the instrument as well as during longer kinetic assays. Instrument has automatic dynamic range selection to adjust the photomultiplier tube sensitivity based on the signal strength of the sample well. Adjustment is done individually for every well and every measurement. Dynamic range for the fluorescence and luminescence should be mentioned and approximately it should be more than 6 to 7 decades. The quoted instrument should have option for field upgradation of luminescence and dispensers for flash luminescence assays like Ca2+ flux , ATP assays etc. Onboard Incubator and shaker should be available. Incubation temperature should be upto 450C and Orbital shaker with adjustable speed and diameter. System should be supplied with Analysis software with unlimited user license. Single software program should allow any number of measurement steps (different detection modes) within the program. Orbital Shaking with adjustable timing, speed and diameter. Automatic safety control based on the shaking speed and plate format to avoid spilling of the liquid from wells. Should be compatible for low volume sample analysis using accessory plate, volumes down to 2 µL. Automatic Smart Safety Checks like Plate check, Prime check, Position sensors, Shaker check and dispensing volume check. Ability to include multiple plates inside a measurement session, and combine data from all plates to the same data set. The instrument controls shaking speed and diameter combinations to help prevent accidental spilling of the liquid out from the wells. Optical System: Instrument should have Quadruple Monochromator based , double excitation and double emission monochromators for fluorescence 1 No. TENDER-917/AMENDMENT-0/19-Jul-2023 12:41:03 PM Page 6 of 11 Seal & Signature of the Vendor applications. Instrument should have double monochromators for photometric (UV and Vis) measurement. The instrument should have a single lamp source and separate detectors for Photometry, Fluorometry and optional module for Time resolved fluorescence and Luminescence. Absorbance / Photometry: Measurement range in Photometry: 200-1000nm Fluorescence/Fluorometry : Fluorometry wavelength selection: Excitation range: 200-1000nm, Emission: 270-840nm. Fluorescence intensity sensitivity of 0.4 fmol fluorescein per well with 384 well black plates. Plate Type : 6 well to 1536 well format Luminometry : Luminometric upgration should be done at field / at Lab. Luminometric sensitivity of <7 amol ATP/well with 384 well white plate using flash ATP reaction. Should have spectral scanning option. Luminometry should have three measurements mode – Normal, Filter and Monochromator mode with excellent sensitivity. Data Analysis Software : Database based software to run backups of all data, restore back up data (in case of hardware failure of original computer. Software should have option for area selection. i. e different protocols at different area of the same plate. Spectral scanning of all 96 samples or 384 samples should be able to view in single graph plot. Single software program should allow any number of measurement steps / different detection method within the same program. Imaging system: System should be Inverted Fluorescence based Automated Cell Imaging System with upgradability to Live Cell Imaging Classification: Should be a high performance automated system for inverted multi-channel fluorescence and transmitted light (bright field and phase contrast) cell imaging applications Illumination: Digitally controlledLED light sources for transmitted & fluorescent light applications with greater durability (≥45,000 hours lifespan). Should include fluorescent LED light sources with hard coated bandpass optical filters for DAPI, GFP and TX Red. The illuminators should have independent intensity control and system should be upgradable upto four fluorescent channels Objectives: Should have infinity corrected optics. Five Position objective turret. Plan Fluorite objectives – 10X, 20X, 40X & 60X should be included Condenser: Should have bright-field and phase contrast annuli. Working distance 60 mm or more Stage: Motorized X-Y scanning stage, travel range 120mm x 80 mm travel stage with submicron resolution. Should becompatible with all commonly used cell culture vessels (viz. slides, flasks, petri dishes, multi-well plates etc.). It should have option to upgrade with onstage incubator for live cell time lapse imaging with precise control of temperature, humidity, gas flow (viz. CO2, air) Camera: Should be integrated with dual cameras - High sensitivity 3.2MP (2,048 x 1,536) monochrome CMOS sensor with 3.45 µm pixel resolution; High-sensitivity 3.2MP (2,048 x 1,536) color CMOS sensor with 3.45 µm pixel resolution with auto switching capabilities. Camera should support autofocusing to optimize speed and accuracy Display: Should be provided with 23-inch high-resolution touch screen LCD color monitor (1,920 x 1,080 resolution) and other relevant accessories Interface: USB ports for connecting with PC Software: Should have integrated software with intuitive GUI for operation TENDER-917/AMENDMENT-0/19-Jul-2023 12:41:03 PM Page 7 of 11 Seal & Signature of the Vendor Captures publication quality images in JPEG/TIFF/PNG formats Compatible with single click multi-channel overlay High-speed image acquisition coupled with multi-position well-scanning. Should be able to scan 96-well plate in three fluorescent channels in less than 10 min Should have a vessel selection wizard and vessel map under live mode Should have features for time-lapse imaging and creating videos in AVI formats Z-stacking feature to capture a series of images along the Z-axis that can be saved individually or combined into a Z-stack projection with a greater depth of field Should have tool for image stitching and montage, cell count, time-lapse live cell imaging Automated scanning & imaging capabilities in defined region (e.g. fields & wells) in different vessel options with at least 4 fluorescent channels & transmitted light applications (Bright-field & Phase Contrast) Data Processing Unit: External PC with 16 GB RAM and instrument having intel core i7 processor, 1 TB hard disk, Optical mouse, keyboard, DVD writer, 23 high‐resolution color monitor (also fully controllable via mouse); 1920 x 1080 pixel resolution, 1 GB NVIDIA Graphic Card, Multimedia kit, 64 bit Windows as. Options for USB and networking support and storage. Vessel Holders: Vessel holders for glass slides, T-25/T-75 Flasks, 35,60, 100 mm petridishes, 96/384 multi-well plates must be included along with the imaging system
