Bids Are Invited For 1 Recombinant Zika Virus Ns1 Protein (His Tag) 2 Anti-Zika Virus Ns1 Protein Antibody 3 Recombinant Zika Virus Envelope Protein (His Tag) 4 Recombinant Anti-Zika Virus Envelope Antibody 5 Glassy Carbon Working Electrode 6 Chitos, Madurai-Tamil Nadu

Bids Are Invited For 1 Recombinant Zika Virus Ns1 Protein ( His Tag ) 2 Anti-Zika Virus Ns1 Protein Antibody 3 Recombinant Zika Virus Envelope Protein ( His Tag ) 4 Recombinant Anti-Zika Virus Envelope Antibody 5 Glassy Carbon Working Electrode 6 Chitosan Powder 7 Magnetic Nanoparticle ( 15Nm, 0.01% Au ) 8 Screen Printed Electrode Ref Electrode: Ag / Agcl, Work: Carbon Ref Electrode:0.5Mm Dimensions: 50 X 10Mm, Thick:0.5Mm 9 Dna Sanger Sequencing: Pcr Product And Gel Purification ~1.5Kb, Forward And Reverse Directions, Linear Amplification. 50 Samples Will Be Send In Batches ( 5-10 Samples / Batch ) Timeline:Samples Will Be Sent Within 6 Month After Awarding Gem Contract. The Results Should Be Given Within 4 Weeks From The Date Of Receipt Of The Samples. 10 Coxiellaburnetii Real Time Pcr Primers And Probe ( Taqman Hydrolysis Probe - 25Nmole ) Cox-F ( 5′-Gtcttaaggtgggctgcgtg-3′ ) Cox-R ( 5′-Ccccgaatctcattgatcagc-3′ ) , Cox-Tm ( Fam-Agcgaaccattggtatcggacgtt-Tamra ) 11 Whole Genome Sequencing By Short And Long Read ( Illumina And Promethion ) ; Whole Genome Sequencing Specifications – Hybrid Sequencing Service Required: Whole Genome Sequencing, Hybrid Sequencing Based On Reference Genome; Organism: Fleas Number Of Samples: 2 Different Species Genome Size: ~ 750 Mb; Isolation Of Dna Required: Yes – High Molecular Weight Platform Required: Illuminanovaseq 6000 And Promethion; Data Required:Short Read – 100X Coverage- Illumina Long Read: 100X Coverage- Promethion Or Any Other Higher Method; Bioinformatics Analysis Required: No; Analysis Required: Raw Data Qc; The Company Should Have An In-House Bioinformatics Facility. The Facility Must Have Nabl, Cap Or Equivalent. Proof Of Certificate Should Be Provided. Lab Should Preferably Have Lims Facility; Data Should Be Of Good Quality Reads I.E., &>90% Data With Phred Score Q> 30; Read Length And Read Type- 2*150 Bp Paired End; File Format: Fastq, Fast5; Data Delivery Through Ssd Timeline:Samples Will Be Sent Within One Week After Awarding Gem Contract. The Results Should Be Given Within 4 Weeks From The Date Of Receipt Of The Samples. 12 Ampliqon Red Dye Master Mix 2X With 1.Mm Mgcl2 13 Ampliqon Multiplex Tempase 2X Master Mix With 25Mm Mgcl2 14 Qiagendneasy Blood And Tissue Kit ( Blood And Tissue Kit ) ( Cat. No: 69506 / Qiagen ) Total Quantity : 313

Sr No	CorrigendumDate	Corrignedum	CorrigendumType	NewSubmissionDate
1	10-10-2023			13-10-2023
2	14-09-2023			22-09-2023
3	22-09-2023			30-09-2023
4	27-09-2023			10-10-2023

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