NGS based Transcriptome sequencing of plant samples (De Novo, Lentil),:RNA isolation, RNA quality analysis of the final RNA used for sequencing in Bioanalyzer followed by transcript Sequencing & Analysis of plant samples involving the cost of total RNA libraries (coding and non-coding) preparation and sequencing at least 30GB data in Illumina Novaseq6000; 100-150bp paired end reads per sample and at least 80% of the sequenced bases should be of Q30 value. Sequenced data with FASTQ files should be uploaded on the FTP server for download and review; other file formats required are: SOFT formatted family files, MINiML formatted family files and Series Matrix Files. Comprehensive bioinformatics analysis of sequenced data should include: a. The Raw fastq files and QC report containing the read information, data size, average base quality, GC percentage, Base quality distribution. b. 5’and 3’read bias should be measured. c. Aligned reads distribution and splice junction information d. Genes and isoforms expression values e. List of genes differentially expressed globally. f. GO annotation for differentially expressed genes such as biological process, molecular function and cellular component, pathway information (KEGG &Reactome). g. Gene Set Enrichment Analysis. h. Cluster analysis and heat maps. i. Publication and training support. j) Additional Analysis as per instruction for the next six months after completion of the project. The servicing laboratory must be CAP/NABL/DSR. Free pick up of sample in dry ice from BCKV must be assured in the quotation.
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