Supply Of Mouse Peroxiredoxin Mouse Peroxiredoxin As Per Annexure Attached . , Mouse Peroxiredoxin 5 Lentiviral Activation Particles, Pack Of 200 Microlitres Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Target-Specific 20 Nucleotide Guide Rna, Provides Robust Recruitment Of Transcription Factors For Highly Efficient Gene Activation Of Prdx5, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Double Nickase Plasmid, Pack Of 20 Micrograms, Suitable For Up To 20 Transfections, Consists Of A Pair Of Plasmids Each Encoding A D10a Mutated Cas9 Nuclease And A Target-Specific 20 Nt Guide Rna ( Grna ) Designed To Knockout Gene Expression With Greater Specificity Than Its Crispr / Cas9 Ko Counterpart, Paired Grna Sequences Are Offset By Approximately 20 Bp To Allow For Specific Cas9-Mediated Double Nicking Of The Genomic Dna, Which Mimics A Dsb; Antibiotic And Gfp Marker On Plasmids For Selection And Visual Confirmation [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Mouse Peroxiredoxin 5 Lentiviral Inactivation Particles, Pack Of 200 ?L Viral Stock Containing 1000000 Infectious Units Of Virus ( Ifu ) , Provided As Transduction-Ready Viral Particles For Gene Silencing, Specific For Mouse Prdx5, Contain Three To Five Expression Constructs Each Encoding Target-Specific 19-25 Nt ( Plus Hairpin ) Shrna Designed To Knockdown Gene Expression, Puromycin Or Similar Antibiotic Selection Marker, Replication-Incompetent And Are Designed To Self- Inactivate After Transduction And Integration Of Shrna Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , 1, 5-Dimethyl-1, 5-Diazaundecamethylene Polymethobromide, Pack Of 1 Ml, Concentration Of 10 Mg / Ml, Infection Reagent Used To Introduce Retroviral Vectors Into Mammalian Cells, Meant To Be Used For Cell Culture By Manufacturer [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Negative Control Lentiviral Activation Particles, Pack Of 200 Microlites Of Transduction-Ready, High-Titer Crispr / Dcas9 Lentiviral Activation Particle, Based On Synergistic Activation Mediator ( Sam ) Transcription Activation System Consisting Ofdeactivated Cas9 ( Dcas9 ) Nuclease ( D10a And N863a ) Fused To The Transactivation Domain Vp64, An Ms2-P65-Hsf1 Fusion Protein And A Non-Specific 20 Nucleotide Guide Rna, Sam Complex Does Not Recognize Or Bind Any Specific Sequence Within The Genomic Dna, And Does Not Activate Transcription Of Any Specific Gene, Must Be Replication-Incompetent And Are Designed To Self-Inactivate After Transduction [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ] , Control Lentiviral Particles With Gfp Coding Construct To Express In Mammalian Cells, Pack Of 200 Microlites Of Transduction-Ready Viral Particles Sufficient For 10 Or More Transductions, Stable Expression Of Gfp After Transduction, Meant To Measure Transduction Efficiency In Cell Type, Replication-Incompetent And Are Designed To Self-Inactivate After Transduction And Integration Of Gfp Control Constructs Into Genomic Dna Of Target Cells [ Vendor Should Provide Catalogue Number / Specification Sheet Of Product That Vendor Wishes To Supply To Allow Us To Cross Check Specifications ]
