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| 1 | List of Chemicals required for Biochemistry Practical Laboratory |
| 2 | Acetic Acid (500ml) |
| 3 | Acetone (2.5 Litre) |
| 4 | Acryl amide (500gm) |
| 5 | Agarose, low EEO(500gm) |
| 6 | Alpha-Napthol(500ml) |
| 7 | Ammonium Acetate (500gm) |
| 8 | Ammonium chloride (500gm) |
| 9 | Ammonium Molybdate (500gm) |
| 10 | Ammonium persulphate (10gm) |
| 11 | Ammonium solution (500ml) |
| 12 | Ammonium sulphate(500gm) |
| 13 | Barium chloride (500gm) |
| 14 | Benedicts reagent(500ml) |
| 15 | Benzedine solution(500ml) |
| 16 | Beta-Mercaptoethanol (1 00gm) |
| 17 | Bis Acryl amide(500gm) |
| 18 | Bromocresol Green (500gm) |
| 19 | Bromophenol Blue(10gm) |
| 20 | Butanol (01 Litre) |
| 21 | Citric Acid(500gm) |
| 22 | Coomassie brilliant Blue (10 gm) |
| 23 | Copper sulphate (500 gm) |
| 24 | Diethyl Barbituric Acid (50 gm) |
| 25 | Dipotassium EDTA (100 gm) |
| 26 | Dipotassium hydrogen phosphate (500 gm) |
| 27 | Disodium EDTA (100 gm) |
| 28 | Disodium hydrogen phosphate (500 gm) |
| 29 | DNA Ladder 50 bp (50 µg) |
| 30 | DNA Ladder 100 bp (50 µg) |
| 31 | dNTPs set (25mM) |
| 32 | Dimethyl Sulphoxide (100 ml) |
| 33 | Ehrlichs reagent (500 ml) |
| 34 | Ethanol Absolute(2.5 Litre) |
| 35 | Ether (500 ml) |
| 36 | Ethidium bromide (10 gm) |
| 37 | Ferric chloride (500 gm) |
| 38 | Fouchets reagent 500 ml) |
| 39 | Glycerol (500 ml) |
| 40 | Glycine (100 gm) |
| 41 | Hydrochloric Acid (500 ml) |
| 42 | Hydrogen peroxide (1 Litre) |
| 43 | Isopropanol (1 Litre) |
| 44 | Liquid Ammonia (500 ml) |
| 45 | Litmus paper |
| 46 | Magnesium chloride (500 gm) |
| 47 | Methanol(500 ml) |
| 48 | Ninhydrin(100 gm) |
| 49 | Nitric Acid (500 ml) |
| 50 | O-Toluidine solution (500 ml) |
| 51 | Para-Dimethyl aminobenzaldehyde (500 mgm) |
| 52 | Paraffin Film |
| 53 | pH Tablets (pH 4.0)(60 g) |
| 54 | pH Tablets (pH 7.2) (60 g) |
| 55 | pH Tablets (pH 9.0) (60 g) |
| 56 | Phosphotungstic Acid (500 ml) |
| 57 | Picric Acid (500 ml) |
| 58 | Potassium dihydrogen phosphate (500 gm) |
| 59 | Potassium Oxalate(500 gm) |
| 60 | Proteinase K (10 gm) |
| 61 | Silica Gel (500 gm) |
| 62 | Silver Nitrate (10gm) |
| 63 | Sodium Acetate (500 gm) |
| 64 | Sodium Bicarbonate (500 gm) |
| 65 | Sodium Carbonate (500 gm) |
| 66 | Sodium Chloride (500 gm) |
| 67 | Sodium Citrate (500 gm) |
| 68 | Sodium diethyl barbiturate(500 gm) |
| 69 | Sodium Dihydrogen phosphate (500 gm) |
| 70 | Sodium Dodecyl Sulphate (1000 gm) |
| 71 | Sodium Hydroxide (500gm) |
| 72 | Sodium Hypobromite (500gm) |
| 73 | Sodium Hypochlorite (500ml) |
| 74 | Sodium Nitroprusside (500 gm) |
| 75 | Stannous Chloride (500 gm) |
| 76 | Sucrose (500 gm) |
| 77 | Sulphosalicylic acid (500 gm) |
| 78 | Sulphur powder (500 gm) |
| 79 | Sulphuric Acid ((500 gml) |
| 80 | Taq DNA polymerase with proof reading activity (1000 U) |
| 81 | TEMED(100 ml) |
| 82 | Trichloroacetic Acid (500 gm) |
| 83 | Tris Base(500 gm) |
| 84 | Triton X 100 (1 Litre) |
| 85 | Whatman filter paper grade 1 |
| 86 | Whatman filter paper grade 2 |
| 87 | Whatman filter paper grade 3 |
| 88 | Xylene cyanol (10gm) |
| 89 | List of Glassware/Plasticware required for Biochemistry Practical Laboratory |
| 90 | Beakers-100 ml |
| 91 | Beakers-250 ml |
| 92 | Beakers-1000 ml |
| 93 | Beakers-500 ml |
| 94 | Capillary tubes |
| 95 | Centrifuge Tubes (15 ml) - 100 per Pack |
| 96 | Centrifuge Tubes (50 ml) - 100 per Pack |
| 97 | China Dish |
| 98 | Chromatography Chamber |
| 99 | Conical Flasks-250 ml |
| 100 | Conical Flasks-500 ml |
| 101 | Filter Paper For Paper Chromatograph- 24 per Pack |
| 102 | Glass Funnels |
| 103 | GraduatedMeasuring Cylinders-1000 ml |
| 104 | GraduatedMeasuring Cylinders-250 ml |
| 105 | GraduatedMeasuring Cylinders-500 ml |
| 106 | Graduated Measuring Cylinders-100 ml |
| 107 | Microcentrifuge tube box for 1.5 ml tubes |
| 108 | Micro-Centrifuge Tubes (1.5 ml) – 1000 per Pack |
| 109 | Micropipette Tip box for 10 µl tips |
| 110 | Micropipette Tip box for 1000 µl tips |
| 111 | Micropipette Tip box for 200 µl tips |
| 112 | Micropipette Tips (10 µl) - 1000 per Pack |
| 113 | Micropipette Tips (1000 µl) - 1000 per Pack |
| 114 | Micropipette Tips (200 µl) - 1000 per Pack |
| 115 | Motor And Pestle |
| 116 | Pasteur Pipettes – 100 per Pack |
| 117 | Reagent Storage BottlesWith Screw Cap-250 ml |
| 118 | Reagent Storage BottlesWith Screw Cap-500 ml |
| 119 | Reagent Storage Bottles With Screw Cap-1000 ml |
| 120 | Spirit Lamps |
| 121 | Test Tube Holders |
| 122 | Test Tube Racks |
| 123 | Test Tubes (20ml) – 100 per pack |
| 124 | Test Tubes (50ml) – 100 per pack |
| 125 | TLC Chamber |
| 126 | TLC Plates |
| 127 | Tripod Stand |
| 128 | Volumetric Flasks-100 ml |
| 129 | Volumetric Flasks-1000 ml |
| 130 | Volumetric Flasks-250 ml |
| 131 | Volumetric Flasks-50 ml |
| 132 | Volumetric Flasks-500 ml |
| 133 | Wash Bottles |
| 134 | Wire Guaze |
| 135 | List of Kits(Kits should be compatible with semi-automatic analyzer) with Methods and Pack Size |
| 136 | Albumin*(Bromocresol Green)(5×25ml) (1×2ml) |
| 137 | ALP*(Paranitrophenyl-p)(425ml) (1×25ml) |
| 138 | Bilirubin*(Diazonium)(5×25ml) (1×2.5ml) |
| 139 | Calcium*(Cal-Arsenazo III)(1×50ml) (1×2ml) |
| 140 | Creatinine* (Jaffe’s method)(4×25ml) (1×25ml) (1×2ml) |
| 141 | CSF protein* (Pyrogalol Red)(6×25ml) |
| 142 | Direct bilirubin*(Diazonium)(5×25ml) (1×2.5ml) |
| 143 | DNA extraction kit from tissue/blood (50 reactions) |
| 144 | Glucose* (GOD-POD)(6×100ml) (1×2ml) |
| 145 | HDL* (PEG – Chod/ PAP (Enzymatic)(2×18.7ml) (6.25×2ml) (1×2ml) |
| 146 | Phosphorus* (Phisphomolybedate) (1×50ml) (1×2ml) |
| 147 | SGOT*(UV-Kinetic Method) (4×25ml) (1×2ml) |
| 148 | SGPT* (UV-Kinetic Method)(4×25ml) (1×2ml) |
| 149 | Standard Amino Acid kit of 24 amino acids |
| 150 | Total Cholesterol* (CHOD-POD)(5×25ml) (1×2ml) |
| 151 | Total Protein* (Biuret)(5×25ml) (1×2ml) |
| 152 | Triglycerides* (Glycerol Phosphate Oxidase)(5×25ml) (1×2ml) |
| 153 | TSH (ELISA based)(96 reactions) |
| 154 | Urea* (Berthelot/Urease)(4×25ml) (1×25ml) (1×2ml) |