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Tender For Sgrna Synthesis Kit, trombay-Maharashtra

Directorate Of Purchase And Stores has published Tender For Sgrna Synthesis Kit. Submission Date for this Tender is 29-11-2022. Water Treatment Consumables Tenders in trombay Maharashtra. Bidders can get complete Tender details and download the document.




Tender Notice

34614059
Tender For Sgrna Synthesis Kit
Open Tender
NCB
Maharashtra
Trombay
29-11-2022

Tender Details

Sgrna Synthesis Kit Supply Of Sgrna Synthesis Kit , Sgrna Synthesis Kit. Rapid Generation Of Microgram Quantities Of Sgrna Of The S. Pyogenes Crispr-Cas9 Systemin 30 Minutes. Complete Workflow Of Less Than One Hour. Simple Protocol Requires Only A User-Supplied ~55 Nucleotide Ssdna Target-Specific Oligonucleotide. The Kit Must Contain S. Pyogens Cas9 Partial Scaffold. The Dsdna And Sgrna Must Be Synthesized In A Single Reaction. Dnase Must Be Supplied With The Kit.The Vendor Should Be An Authorized Dealer For The Product And The Catalogue Of The Material Must Be Provided With The Quote. , Rt-Warm Start Lamp Kit, 500 Reactions. Simple, One-Step Solution For Loop-Mediated Isothermal Amplification ( Lamp ) Of Dna Or Rna ( Rt-Lamp ) Targets.Kit Should Be Supplied With The Warmstart Lamp 2X Master Mix, Which Contains A Blend Of Bst 2.0 Warmstart Dna Polymerase And Warmstart Rtx Reverse Transcriptase In An Optimized Lamp Buffer Solution.The Vendor Should Be An Authorized Dealer For The Product And The Catalogue Of The Material Must Be Provided With The Quote. , Rt-Warm Start Lamp Kit ( With Udg ) 100 Reactions. Simple, One-Step Solution For Loop-Mediated Isothermal Amplification ( Lamp ) Of Dna Or Rna ( Rt-Lamp ) Targets.Kit Should Be Supplied With The Warmstart Lamp 2X Master Mix, Which Contains A Blend Of Bst 2.0 Warmstart Dna Polymerase And Warmstart Rtx Reverse Transcriptase In An Optimized Lamp Buffer Solution. The Kit Should Include Dutp And Thermolabile Udg In The Master Mix To Reduce The Possibility Of Carryover Contamination. The Vendor Should Be An Authorized Dealer For The Product And The Catalogue Of The Material Must Be Provided With The Quote. , Pcr & Dna Cleanup Kit. 50 Preparations. For Purification Of Up To 5 ?G Of Concentrated, High-Quality Dna From Pcr And Other Enzymatic Reactions. The Kit Should Utilize A Bind / Wash / Elute Workflow With Minimal Incubation And Spin Times. Eluted Dna Should Be Ready For Use In Restriction Digests, Dna Sequencing, Ligation And Other Enzymatic Manipulations. Should Allow Purification Of Ssdna Oligonucleotides ( = 18 Nt ) And Dsdna Fragments ( = 15 Bp ) . The Columns Should Have Zero Retention Of Buffer And No Carryover Of Buffers. Typical Yields Must Be In The Following Range: Dna ( 50 Bp To 10 Kb ) : 70–90% Dna ( 11–23 Kb ) : 50–70% Ssdna =18 Nt And Dsdna = 15 Bp: 70-85%. The Vendor Should Be An Authorized Dealer For The Product And The Catalogue Of The Material Must Be Provided With The Quote. , T4 Dna Ligase, 20000Units. For Ligating Dna / Rna Fragments Together.Cloning Of Restriction Fragments Joining Linkers And Adapters To Blunt-Ended Dna. Should Have No Detectable Endonuclease Or Exonuclease Contamination After 4H Of Incubation With Dna At Reccommended Concentration. The Vendor Should Be An Authorized Dealer For The Product And The Catalogue Of The Material Must Be Provided With The Quote.

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